Biogenic Synthesis of Silver Nanoparticles Using Fruit Aqueous Extract of Psidium Guajava and Its Antibacterial Activity

Document Type : Original Research Paper


Department of Chemistry, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran


One plant seed extract (Psidium guajava) was screened for their bioreduction behavior for synthesis of silver nanoparticles. Psidium guajava (PG) was found to exhibit the good reducing and protecting action in terms of synthesis rate and monodispersity of the prepared silver nanoparticles. UV–visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and X-ray energy dispersive spectrophotometer (EDAX) was performed to ascertain the formation of Ag-NPs. Our measurements indicate that biosynthesis of Ag nanoparticles by Psidium guajava (PG) produces Ag nanoparticles with the diameters in the range of 30-36 nm. XRD studies reveal a high degree of crystallinity and monophasic Ag nanoparticles of facecentered cubic (FCC) structure. The FTIR result clearly showed that the extracts containing OH as a functional group act in capping the nanoparticles synthesis. Antibacterial activities of Ag-NPs were tested against the growth of Gram-positive (S. aureus) using SEM. The inhibition was observed in the Ag-NPs against S. aureus. The results suggest that the synthesized Ag-NPs act as an effective antibacterial agent. It is confirmed that Ag-NPs are capable of rendering high antibacterial efficacy and hence has a great potential in the preparation of drugs used against
bacterial diseases.


1. Y. Sun, B. Mayers, T. Herricks, Y. Xia, Nano Lett. 3 (2003) 955.
2. H. Srikanth, R. Hajndl, C. Chirinos, J. Sanders, Applied Physics Letters 79 (2001) 3503.
3. M Valle-Orta, D Díaz, P Santiago, E Reguera, A Vázquez-Olmos, Journal of Physical Chemistry 2008 (B 112) 14427.
4. L. Guo, Q. Huang, X. Li, S. Yang, Phys. Chem. Chem. Phys. 3 (2001) 1661.
5. A. Alqudami and S. Annapoorni, Plasmonics 2, 7 (2007) 5.
6. S.S. Shankar, A. Rai, A. Ahmad, M.Sastry, J.Colloid Interface Sci. 275 (2004) 496.
7. N. Duran, P.D. Marcato, G.I.H. De Souza, O.L. Alves, E. Esposito, J. Biomed. Nanotechnol. 3 (2009) 1.
8. J. Xie, J.Y. Lee, D.I.C. Wang, Y.P. Ting, Small 3 (2007) 672.
9. Y. Zhou, W. Chen, H. Itoh, K. Naka, Q. Ni, H. Yamane, Y. Chujo, Chem. Commun. (2001) 2518.
10. X. Zhai, S. Efrima, J. Phys. Chem. 100 (1996) 10235.
11. M.A. El-Sayed, Acc. Chem. Res. 34 (2001) 257.
12. A. Šileikaite, I. Prosycˇevas, J. Puišo, A. Juraitis, A. Guobiene, Mater. Sci. 12 (2006) 287.
13. I. Sondi, B. Salopek-Sondi, J. Colloid Interf. Sci. 275 (2004) 177.
14. G.A. Sotiriou, S.E. Pratsinis, Sci. Technol. 44 (2010) 5649.
15. J. Huang, G. Zhan, B. Zheng, D. Sun, F. Lu, Y. Lin, H. Chen, Z. Zheng, Y. Zheng, Q. Li, Ind. Eng. Chem. Res. 50 (2011) 9095.
16. L. He, S. Gao, H. Wu, X. Liao, Q. He, B. Shi, Mater. Sci. Eng. C 32 (2012) 1050.
17. S. Kaviya, J. Santhanalakshmi, B. Viswanathan, J. Muthumary, K. Srinivasan, Spectrochim. Acta Part A 79 (2011) 594.
18. E.K. Elumalai, T.N.V.K.V. Prasad, J. Hemachandran, S.V. Therasa, T. Thirumalai, E. David,J. Pharm. Sci. Res. 2 (2010) 549.
19. M. Safaepour, A.R. Shahverdi, H.R. Shahverdi, M.R. Khorramizadeh, A.R. Gohari, J. Med. Biotechnol. 1 (2009) 111.
20. K. Kalishwaralal, V. Deepak, S.R.K. Pandian, M. Kottaisamy, S.B. Manikanth, B. Kartikeyan, S. Gurunathan, Colloid. Surface. B 77 (2010) 257.
21. M.N. Nadagouda, G. Hoag, J. Collins, R.S. Varma, Cryst. Growth Des. 9 (2009) 4979.
22. M.A.S. Sadjadi, Babak Sadeghi, M. Meskinfam, K. Zare, J. Azizian, Physica E: Low-dimensional Systems and Nanostructures, 40 (2008) 3183.
23. Babak Sadeghi, M.A.S. Sadjadi, R.A.R. Vahdati, Superlattices and Microstructures 46 (2009) 858.
24. Babak Sadeghi,M. Jamali, Sh. Kia, A. Amini Nia, S. Ghafari, Int.J.Nano Dimens. 1 (2010) 119.
25. Babak Sadeghi, Farshid S.Garmaroudi, M. Hashemi, H.R. Nezhad, A. Nasrollahi, Sima Ardalan, Sahar Ardalan, Advanced Powder Technology 23 (2012) 22.
26. Babak Sadeghi, A.Pourahmad, Advanced Powder Technology 22 (2012) 669.
27. Babak Sadeghi, Sh. Ghammamy, Z. Gholipour, M. Ghorchibeigy, A. Amini Nia, Mic & Nano Letters. 6 (2011) 209.
28. Babak Sadeghi, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 118 (2014) 787.
29. Babak Sadeghi, M. Meskinfam, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 97 (2012) 326.
30. B. Umesh, J.Vishwas, A.Bapat, Industrial Crops and Products 46 (2013) 132.
31. Q.L. Feng, J. Wu, G.Q. Chen, F.Z. Cui, T.N. Kim, J.O. Kim, J. Biomed. Mater. Res. 52 (2000) 662.
32. A. Melaiye, Z. Sun, K. Hindi, A. Milsted, D. Ely, D.H. Reneker, C.A. Tessier, W.J.Youngs, J. Am. Chem. Soc. 127 (2005) 2285.
33. W.K. Son, J.H. Youk, T.S. Lee, W.H. Park, Macromol. Rapid Commun. 25 (2004) 1632.
34. M.Sastry, K.S.Mayyaa, K.Bandyopadhyay, Colloids Surf. A 127 (1997) 221.
35. X. Wang, G.J. Bunkers,Biochem. Biophys. Res. Commun. 279 (2000) 669.
36. K. Badri Narayanan, N. Sakthivel, Mater. Lett. 62 (2008) 4588.
37. S. He, Y. Zhang, Z. Guo, N. Gu, Biotechnol. Prog. 24 (2008) 476.
38. D.S. Sheny, Daizy Philip, Joseph Mathew, Spectrochim. Acta A 91 (2012) 35.
39. S. Basavaraja, S.D. Balaji, A. Lagashetty, A.H. Rajasab, A. Venkataraman, Mater. Res. Bull. 43 (2008) 1164.
40. A.Kumar Mittal, Y.Chisti, U.Chand Banerjee. Biotechnology Advances 31 (2013) 346.
41. A.S. Eppler, G. Rupprechter, E.A. Anderson, G.A .Somorjai.J.Phys.Chem.B. 104 ( 2000) 7286.
42. S. S,Shankar, A. Rai, A .Ahmad, M. Sastry.J Colloid Interface Sci. 275 ( 2004) 496.
43. K. Badri Narayanan, N. Sakthivel, Mater. Lett. 62 (2008) 4588.
44. Chaloupka K, Malam Y, Seifalian AM. Trends Biotechnol 28 (2010) 580.