Davari, S., Shirkhanloo, H., Motakef Kazemi, N. (2017). Application of Nanoanalysis: Amine-Functionalized Mesoporous Silica nanoparticles for Mercury Speciation in Human Samples. Journal of Nanoanalysis, 4(3), 181-188. doi: 10.22034/jna.2017.539360
Sara Davari; Hamid Shirkhanloo; Negar Motakef Kazemi. "Application of Nanoanalysis: Amine-Functionalized Mesoporous Silica nanoparticles for Mercury Speciation in Human Samples". Journal of Nanoanalysis, 4, 3, 2017, 181-188. doi: 10.22034/jna.2017.539360
Davari, S., Shirkhanloo, H., Motakef Kazemi, N. (2017). 'Application of Nanoanalysis: Amine-Functionalized Mesoporous Silica nanoparticles for Mercury Speciation in Human Samples', Journal of Nanoanalysis, 4(3), pp. 181-188. doi: 10.22034/jna.2017.539360
Davari, S., Shirkhanloo, H., Motakef Kazemi, N. Application of Nanoanalysis: Amine-Functionalized Mesoporous Silica nanoparticles for Mercury Speciation in Human Samples. Journal of Nanoanalysis, 2017; 4(3): 181-188. doi: 10.22034/jna.2017.539360
Application of Nanoanalysis: Amine-Functionalized Mesoporous Silica nanoparticles for Mercury Speciation in Human Samples
1Department of Medical Nanotechnology, Faculty of Advanced Sciences & Technology, Pharmaceutical Sciences Branch, Islamic Azad University, (IAUPS), Tehran, Iran
2Research Institute of Petroleum Industry (RIPI), West Entrance Blvd., Olympic Village, Tehran, Iran
Abstract
Mercury (Hg) causes hazardous cumulative effects in humans; like central nervous system disorders, hypertension and chromosomal aberrations. Therefore, due to high toxicity and bioaccumulation factor, mercury determination and speciation in human blood is very important. A sensitive, accurate, precise and inexpensive method was demonstrated for preconcentration and speciation of ultra-trace mercury in human blood samples. The amine-functionalized mesoporous silica nanoparticles (NH2-MSNPs) as solid phase sorbent was prepared and investigated in a new and simple dispersive-ionic liquid-micro-solid phase extraction (D-IL-μ-SPE) procedure for rapid speciation and determination of trace inorganic and organic mercury [Hg+2 , (CH3Hg)+ and (C2H5Hg)+] in human blood samples. Under the optimal conditions, the linear range, limit of detection and preconcentration factor were obtained 0.05-9.8 μg L−1, 15 ng L−1 and 9.6 for human blood samples, respectively (Peak Area, RSD<5%). The reusability and batch adsorption capacity of the MSNPs and NH2-MSNPs were also obtained 25 and 159 mg g-1, respectively. The developed method was successfully applied for validation of methodology by standard reference materials (SRM).