A Biotechnological Perspective on The Affinity Magnetic Separation and Purification Based on Oligonucleotides

Document Type : Review Paper


1 Radiation Application Research School, Nuclear Science and Technology Research Institute, P.O. Box 11365-3486, Tehran, Iran

2 Department of Chemistry, Razi University, Kermanshah, Iran

3 Radiation Application Research School, Nuclear Science and Technology Research Institute, Tehran, Iran


The rapidly growing field of biotechnology has created a critical need for simple, fast and
high-throughput processes for the separation and purification of biomolecules from biological
matrices. In recent years, several bioseparation techniques have been proposed as advanced
alternatives to the classical separation methods. These modern processes emphasize ultrahigh
selective and sensitive analysis to determine promising chemical and biological entities.
The current paper discusses the recent developments in the field of biotechnology using
magnetic separation techniques based on oligonucleotides as the chemically synthesized and
cost-effective biological ligands. In particular, they are very stable and not subject to thermal
and chemical degradation. This allows the researchers to use labeled aptamers as highly
sensitive and specific olgonucleotide probes in solution, membrane or magnetic nanoparticlesbased
assay systems. Since aptamers bind their targets with high affinity and specificity, they
are promising alternative ligands in the purification of proteins. The purpose of this review
paper is to show the critical role of oligonucleotides ligands for separation or purification of
biological compounds and ions in complex matrices. Using the suitable aptamers in separation
and preconcentration processes is an excellent alternative to the classical ligands. Some
practical examples of DNA-based magnetic separation processes are also discussed to show
the efficiency of magnetic bioseparation.