Histopathological study on acute toxicity of nanochelating based silver nanoparticles in mouse model

Document Type : Original Research Paper


1 Pediatric Infections Research Center, Research Institute for Children̍s Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran

2 Department of Research and Development, SodourAhrarShargh Company, Tehran, Iran / Cancer Research Center, ShahidBeheshti University of Medical Science, Tehran, Iran

3 Department of Research and Development, SodourAhrarShargh Company, Tehran, Iran



For the therapeutic application and drug delivery of AgNPs in medicine, pharmacy and cosmetic
products, it is essential to know the distribution and local or systemic toxicity associated with them.
For this purpose, this study was carried out to assess the potential consequences of skin injection of
nanochelating based synthesized AgNPs on the mice models. Nanochelating technology used to design
and synthesize the AgNPs. The histopathological findings in skin and tissue of micemodels have assessed
via histopathological analysis.All samples were visualized by an independent pathologist. The results of
each sample have reported as follows, micrograph of the skin cells have shown the normal architecture
and cells in all samples. Moreover, histopathological evaluation of samples have shown normal without
any significant pathological changes compared to control groups in volume of derm and epiderm, and
the number of fibroblast, neutrophil, and macrophage.In summary, this study has observed no obvious
decline of immunological performance and morphological signs of skin damage in the mice caused by
nanochelating based AgNPs exposure. These findings could provide a fundamental understanding of the
intrinsic toxicity associated with nanochelating based AgNPs on biological models. Moreover, this study
would arisetypical attentions on the future applications of nanochelating based AgNPs on human, which
is valuable for short-term and low dose treatment in nanomedicines.