Albumin binding and cytotoxicity assay of nickel oxide nanoparticles against primary hippocampal neural cells

Document Type: Original Research Paper

Authors

1 1Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

2 Department of Nanotechnology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran;

10.22034/jna.2020.674956

Abstract

Nanoparticles (NPs) have been widely used in medical and therapeutic
applications. However, their albumin binding and their cytotoxicity assays have
not been well explored. In his study, the interaction of NiO NPs with human serum
albumin (HSA) was explored by circular dichroism (CD) study, molecular docking
and dynamic studies. Afterwards the cytotoxicity of NiO NPs against primary
hippocampal neural cells was explored by MTT and morphological assays. The
CD experiment revealed that NiO NPs do not stimulate any significant structural
changes within the HSA structure. The molecular docking investigation showed
that NiO nanoclusters bind to HSA molecule through hydrophilic interactions and
NiO nanoclusters with different sizes show different docking scores for interaction
with HSA. The Molecular dynamic study also revealed that minor structural
changes in HSA structure occur after interaction with NiO NPs. Cellular assay
displayed that incubation of NiO NPs with primary hippocampal neural cells for
24 h triggered a significant cytotoxicity and morphological changes. Therefore, it
may be concluded that although NiO NPs may show a strong binding affinity to
HSA and do not induce a remarkable rearrangement in the structure of HSA, they
may induce some unwanted effects on the cell viability

Keywords