Aloe Vera gel extract and sunlight mediated synthesis of silver nanoparticles with highly effective antibacterial and anticancer activity

Document Type: Original Research Paper


1 Department of biophysics , faculty of science , cairo university

2 Biological Evaluation of Natural and Microbial Products Unit, National Research Centre, Dokki, Giza, Egypt



In this study, a single-step method for green synthesis of silver nanoparticles using
Aloe Vera gel extract and sunlight was investigated. The Aloe Vera gel extract
is composed of pectins, lignin, and hemicellulose, which can be used in the
reduction of silver ions to produce colloidal silver nanoparticles (AgNPs-AV). The
preliminary preparation of silver nanoparticles was observed by an immediate
color change to brown. The prepared silver nanoparticles were characterized
by transmission electron microscopy, Fourier transform infrared spectroscopy,
dynamic light scattering, and zeta potential measurements. Additionally, the
cytotoxicity and antimicrobial activity of AgNPs-AV was tested. Results indicated
the formation of stable spherical 90±40nm with a strong absorption peak appears
between 400 and 500 nm. 28.7 ±0.781% of breast cancer cells survived after
incubation with AgNPs-AV invitro for 72 hrs. In addition to AgNPs-AV inhibited
the growth of the most important human skin pathogens (candida albicans,
Pseudomonas aeruginosa and Staphylococcus aureus). The largest inhibition
zone was observed for candida albicans. This method for synthesis is very fast,
produces spherical colloidal silver nanoparticles having an excellent antioxidant
activity, high anticancer therapeutic index and very promising antibacterial
activity. This greenly synthesized nano-formulation has a great potential to be
explored in many different aspects.