Synergistic effects of Titanium dioxide nanoparticles and Paclitaxel combination on the DNA structure and their antiproliferative role on MDA-MB-231cells

Document Type: Original Research Paper

Authors

1 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran

3 Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi, India

10.22034/jna.2020.677396

Abstract

Objective(s): The objective of this investigation was to evaluate the synergistic
effect of paclitaxel (PTX) combined with titanium dioxide nanoparticles (TiO2NPs)
on DNA structure and to examine the proliferation of MDA-MB-231cells.
Methods: This investigation performed with Ultraviolet spectroscopy, zeta
potential investigation, circular dichroism (CD) spectroscopy, ELISA reader and
fluorescence spectroscopy.
Results: The Ultraviolet results indicated that the structure of DNA in the presence
of PTX and TiO2NPs (at a lower concentration) changed significantly rather than
TiO2NPs or PTX alone. The fluorescence results exposed that PTX+TiO2NPs could
form a complex via non-intercalative mechanism and the PTX+TiO2NPs affinity
to DNA increased considerably. The thermodynamics parameters displayed that
PTX+TiO2NPs interact with DNA strongly and in this interaction, the hydrophobic
force plays an important role. The CD data confirmed that DNA structure was
modified by PTX+TiO2NPs via a simple and reasonable mechanism: change in DNA
conformation from B to C-form. The negative charge of DNA reduced strongly
after addition of PTX+TiO2NPs. The anticancer property of PTX+TiO2NPs by
MTT assay demonstrates that this combination can tremendously diminish the
proliferation of MDA-MB-231cells compared to PTX or TiO2NPs alone.
Conclusions: Based on this investigation TiO2NPs could enhance the affinity
and binding of PTX (at a lower concentration) on DNA structure and PTX+NDs
can promote mortality of MDA-MB-231 cells. This study can offer an innovative
strategy for designing the ideal anti-tumor agents.

Keywords