Macro-and micromechanical modelling of HA-Elastin scaffold fabricated using freeze drying technique

Document Type : Original Research Paper


1 Department of Mechanical Engineering, Amirkabir University of Technology, Tehran, Iran

2 New Technologies Research Center, Amirkabir University of Technology, Tehran, Iran

3 School of Dentistry, Marquette University, Milwaukee, USA

4 New Technology Research Center, Amirkabir University of Technology, Tehran, Iran



Since osteomyelitis is a serious and dangerous disease, it requires immediate
treatment with antibiotics or bone substitute replacement in orthopedic surgeries.
Therefore, a porous polymeric-ceramic was fabricated using hydroxyapatite
(HA) and polymethylmethacrylate (PMMA) composed with elastin as an ideal
scaffold for bone tissue engineering applications. The current study is aimed at
investigating the effects of various amounts of elastin biopolymer on porous bionanocomposite
scaffold using the freeze-drying (FD) technique. The morphology
and phase analysis of the prepared scaffold are analyzed using scanning electron
microscope (SEM) and X-ray diffraction (XRD) techniques. The biological
performance of the porous tissue is evaluated in simulated body fluid (SBF) and
sodium chloride (SC) solution. The tensile test is used to measure the elastic
modulus and tensile strength of the porous tissue before soaking in the SBF. The
obtained result is simulated using micromechanical model from the experimental
values. The elastic modulus of samples decreases from 1.18 MPa to 0.69 MPa,
and porosity evaluation is in the range of 70-85% with addition of 10 wt% and 15
wt% elastin to PMMA-HA bio-nanocomposite. The biological behavior indicates
that a thick apatite layer precipitate on the surface of the sample with 10 wt%
elastin beside increases alkaline group with constant pH concentration. According
to the obtained porosity and elastic modulus results, suitable micromechanical
model is assessed. The comparison of micromechanical model is assessed, and
error rate was less than 10%; therefore, optimum model is introduced as the best
micromechanical model for porous bone substitute.