In Vitro Behavior of a Novel Nanostructure Hardystonite/Biphasic Calcium Phosphate Scaffolds (HT/BCPS); Evaluation and Investigation

Document Type : Original Research Paper


Biomaterials Dep, University of Toronto



In this study, highly porous almost (75%) nanostructured Hardystonite/biphasic calcium phosphate scaffolds (BCPS) with interconnected porosity were developed using various hardystonite (HT) contents via space holder technique. Transmission electron microscopy (TEM), X-ray diffraction (XRD), and scanning electron microscopy (SEM) techniques were employed to evaluate different samples. In
addition, the agents of scaffold composition of mechanical behavior, bioactivity, and biodegradability were studied. Also, the results showed that the produced scaffolds had an average pore size and density between 250-350 μm and 2.2 ± 0.4 - 1.7 ± 0.2 gr/cm3, respectively, depending on the Hardystonite (HT) with different contents. Furthermore, increasing the hardystonite content of scaffolds from 0 (control) to 30 wt. % enhanced the bioactivity test, biodegradability, and compressive strength from 1.1 ± 0.1 to 3.1 ± 0.2 MPa, respectively. Besides, MTT assay also confirmed that the BCPS30 (containing 30 wt. % of hardystonite) significantly promoted cell viability and cell adhesion compared to BCPS 0. Totally, our project suggests that nanostructured hardystonite/BCPS with improved biological and mechanical behavior (properties) could potentially be used for biomedical engineering such as bone tissue engineering applications.