A novel investigation on the characterization of bioactive glass cement and chitosan-gelatin membrane for jawbone tissue engineering

Document Type : Original Research Paper


1 Faculty of Biomedical Engineering, Islamic Azad University, Science and Research Branch, Tehran, Iran

2 Assistant professor of biomedical engineering

3 School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran

4 2 School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran 3 Applied Biotechnology Research Center, Baqiyatallah University of Medical Science, Tehran, Iran.

5 4 Dental School, Shiraz University of Medical Sciences, Shiraz, Iran.



The aim of this work was to investigate the characterization of a bioactive glass
(BG) ceramic prepared by sol-gel technique and chitosan-gelatin membranes
prepared by lyophilization technique containing 0, 1, and 2 vol.% ethanol. Earlyabsorbing
and late-absorbing bone substitutes in practice constitute the main
volume of bone substitutes used by dentists and orthopedic surgeons. When
the graft is to be gradually replaced by normal bone, the important issue is the
duration and rate of graft absorption. The prepared samples were characterized
using Fourier transformed infrared spectroscopy (FTIR) and X-ray diffraction (XRD)
analysis. The presence of Ag nanoparticles in bioactive glass was evaluated.
Porous membranes were examined under scanning electron microscopy (SEM)
to estimate the size of the pores and analysis the morphological behavior. The
pore diameter could be controlled within the range of 10–30 μm by adjusting the
percentage of ethanol. The formation of needle-like hydroxyapatite (HA) crystals on
the surface of the membrane after 7 days of immersion in the ringer's solution was
also assessed using SEM images. The SEM image results illustrated the porous
structures in the membranes. The average pore size for chitosan-gelatin membrane
with 2% ethanol was 30±6.3 μm. Finally, these obtained results suggest that
the developed membrane with 2% ethanol possesses the prerequisites for tissue
engineering and can be used for jawbone tissue engineering applications.